CESG Structural Genomics Platform Flowchart

After target selection, the process of structural determination began with cloning sequencing. This sequencing information was sent to external databases like GenBank, PDB, and BMRB. The target for clone sequencing can either be as result of target selection or it can be from a cDNA library or clone library. After the target passed through cloning sequencing, it was processed by small-scale screening to test the likelihood it would pass through stages downstream. Following small-scale expression testing, the target was sent to large-scale production, followed by screening for suitability. If the protein passed this point, its structure was determined, refined, and later validated. At each of these checkpoints listed above, experimental information was deposited into Sesame (LIMS) program. Finally, the validated structure and associated data was used for futher functional studies and / or is published.

Targets progress through CESG's functional teams, in the following order, shown below, at left and at right, and can pass, or fail, at numerous points. Click on the team name to learn more about each team:


Bioinformatics

Cloning

Expression Testing

Cell-Based Protein Production

Cell-Free Protein Production

Protein Purification

Quality Assurance

Nuclear Magnetic Resonance

X-ray Crystallography

Sesame (LIMS)